The sorter is a FacsAria called Sorp specially designed for research.
It is equipped with 5 excitation sources (355, 405, 488, 561, 633 nm) and a PMT on the FSC size parameter allowing the detection and sorting of microparticles / microvesicles. A real UV laser has been installed for a better detection of stem cells of “Side Population” phenotype.
19 parameters are available including 16 in fluorescence (PMT) + one PMT in FSC (microparticles / microvesicles > 0,2μm).
This sorter is dedicated to the sorting of L1 and L2 class cells that do not contain human pathogens.
Optical configuration :
| Laser | Puissances* | Filtres** | Fluorochromes |
| Genesis UV/355 nm | 100mW | BP450/50 LP670 |
Hoechst 33342 bleu, Dapi, Indo-1 Hoechst 33342 rouge |
| Obis 405 nm | 100mW | BP450/50 BP525/50 BP610/20 BP670/30 |
Brillant Violet 421, BD Horizon V450, Pacific Blue, CFP, SYTOX Blue, eFluor450, DAPI Brillant violet 510, BD Horizon V500, AmCyan, Qdot525 Brillant violet 605, Qdot605, BD Horizon 605 Brillant violet 650, Qdot655 |
| SapphireHP 488 nm | 100mW | BP488/10 BP530/30 BP695/40 |
SSC FITC, Alexa Fluor 488, GFP, YFP, SYTOX Green PerCp, PerCp-Cy5-5, IP, 7AAD |
| SapphireHP 561 | 100mW | BP585/15 BP610/20 BP670/30 BP710/50 BP780/60 |
PE, DsRed, dTomato PE-texas red, IP, mCherry, mRasberry, mStrawberry, mRFP PE-Cy5, 7AAD, PE-Cy5.5 Pe-Alexa 700 PE-Cy7 |
| Obis 640 nm | 40mW | BP670/30 BP730/45 BP780/60 |
APC, Alexa Fluor 647, SYTOX Red Alexa Fluor 700 APC-Cy7, APC-H7 |
*Laser power adjustable by software function
** Interchangeable dichroic filters and mirrors
Applications
- 1 to 4-way sorting in micro-tubes, Eppendorf, 12×75 mm and 15ml. In manual settings, possibility to sort on a 96-well plate
- Controlled temperature of the samples (4ºC ,20ºC ,37ºC or 42ºC)
- Stem cells (Side population: Exclusion of Hoechst 33342)
- Sorting in 6/12/48/96 well plates
- Cloning of populations in 96/384-well plates with phenotypic characterisation (BD Index sort),