This analyser is equipped with 4 excitation sources, a PMT on the FSC size parameter allowing the detection of microparticles / microvesicles (>0.2µ) and an SSC on the 405nm laser. A real UV laser has been installed for a better detection of “Side Population” phenotype stem cells.
- 4 lasers: Ultraviolet (355nm/UV), Violet (405nm), Blue (488nm) and Red (635nm)
- Multicolour analysis up to 19 parameters including 16 fluorescence (PMT)
- Acquisition speed up to 20,000 events/second
This device is dedicated to the analysis of L1 and L2 class cells that do not contain human pathogens.
Optical configuration
Laser | Puissances* | Filtres** | Fluorochromes |
Genesis UV/355 nm | 20mW | BP450/50 LP670 |
Hoechst 33342 bleu, Dapi, Indo-1, BUV395 (BP390/18**) Hoechst 33342 rouge |
Coherent Cube 405 nm | 50mW | BP405/2 BP450/50 BP525/50 BP610/20 BP670/30 BP710/50 BP780/60 |
SSC BV421, BD Horizon V450, Pacific Blue, CFP, eFluor450 BV510, BD Horizon V500, AmCyan, Qdot525 BV605, Qdot605, BD Horizon 605, eFluor605 BV650, Qdot655, eFluor650 BV711 BV786 |
Coherent Sapphire 488 nm | 50mW | BP488/10 BP530/30 BP575/26 BP610/20 BP695/40 BP780/60 |
SSC FITC, Alexa Fluor 488, GFP, SYTOX Green, YFP PE PE-CF594, PE-texas red, IP PerCp, PE-Cy5, PE-Cy5.5, PerCp-Cy5-5, IP, 7AAD, PerCP-eFluor710 PE-Cy7 |
Coherent Cube 635 nm | 40mW | BP670/30 BP730/45 BP780/60 |
APC, Alexa Fluor 647, SYTOX Red Alexa Fluor 700 APC-Cy7, APC-H7, Alexa Fluor 750, DRAQ5 |
*Laser power adjustable by software function
** Interchangeable dichroic filters and mirrors
BV: Brillant Violet, SB: Super Bright